Validation of a radioimmunoassay for measuring testosterone concentrations in plasma samples of the subterranean rodent Ctenomys talarum...

VERA FEDERICO ; ZENUTO ROXANA RITA; ANTENUCCI CARLOS DANIEL; BUSSO JM; MARIN RH

JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2011 p. 572 - 583

1932-5223

We validated the Coat-a-Count® radioimmunoassay (RIA) kit for measuring testosterone in plasma samples of the subterranean rodent Ctenomys Talarum and evaluated testosterone levels in free-living and captive individuals. The performance of the assay was evaluated by the assessment of parallelism, accuracy and precision. Moreover, the high specificity of the assay antibody was confirmed by means of a high pressure liquid chromatography with ultraviolet detector (HPLC-UV), followed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Results indicated that plasma samples have to be treated with heat and diluted before the RIA for the optimization of the assay. Plasma testosterone concentrations in free-living animals were outstandingly-elevated (up to 329 ng/mL), which are among the highest ever reported for mammals. On the other hand, captivity produced a 14-fold decrease in plasma testosterone concentrations, emphasizing that very significant changes in the endocrine milieu may occur in wild animals kept under laboratory conditions. Our results place tuco-tucos as an interesting model for the study of androgen regulation in mammals, suggesting that target tissues may have low sensitivity to the testosterone signal and agree with a scenario of elevated levels of sex-hormone binding globulin SHBG) in plasma.