”Inhibition of the growth of Paenibacillus larvae the causal agent of American Foulbrood of honey bees by selected strains of aerobic spore forming bacteria isolated from apiarian sources”

ALIPPI, A. M. & REYNALDI, F. J.

JOURNAL OF INVERTEBRATE PATHOLOGY

Elsevier

Lugar: San Diego. USA; Año: 2006 vol. 91 p. 141 - 146

0022-2011

The bacterium Paenibacillus larvae, the causative agent of American Foulbrood disease of honey-bee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and other apiarian sources were evaluated. Each isolate was screened against one strain of Paenibacillus larvae (ATCC 9545) by using a perpendicular streak technique. Ten randomly selected bacterial strains from the group  that showed the best antagonistic effect to P. larvae ATCC 9545 were selected for further study. These were identified as Bacillus subtilis (m351), Bacillus pumilus (m350), Bacillus licheniformis (m347), Bacillus cereus (mv33), Bacillus cereus (m387), Bacillus cereus (m6c), Bacillus megaterium (m404), Brevibacillus laterosporus (BLAT169), Brevibacillus laterosporus (BLAT170), and B. laterosporus (BLAT171). The antagonistic strains were tested against 17 P. larvae strains from different geographical origins by means of a spot test in wells. The analysis of variance and posterior comparison of means by Tukey method (P < 0.01 ) showed that the best antagonists were Bacillus megaterium  (m404), Bacillus licheniformis (m347), Bacillus cereus (m6c), B. cereus (mv33), and B. cereus (m387).