Hsp27 (HSPB1): a possible surrogate molecular marker for loss of heterozigocity (LOH) of chromosome 1p in oligodendrogliomas but not in astrocytomas.

CASTRO G. N.; CAYADO-GUTIÉRREZ N.; MONCALERO V. M.; LIMA P.; LUCERO DE ANGELIS R.; CHAVEZ V.; CUELLO CARRIÓN F. D.; CIOCCA D. R.

CELL STRESS & CHAPERONES.

SPRINGER

Lugar: Berlin; Año: 2012 vol. 17 p. 779 - 790

1355-8145

In oligodendrogliomas, 1p LOH  is a predictor of good prognosis and treatment response. In contrast, in uveal melanomas LOH of chromosome 3 has been linked to poor prognosis and down-regulation of Hsp27. In the present study we have  analyzed  the expression of  heat shock proteins (Hsps)  to characterize subtypes of gliomas and their histopathologic features, and  to correlate with other molecular markers including LOH of 1p. Biopsies from patients with primary gliomas  (n=65) were analyzed by  immunohistochemistry, CISH and FISH and methylation specific PCR (MSP). Elevated Hsp27 and Hsp70t  expression  levels were associated with high  grade astrocytomas  (p=0.0001  and p=0.01 respectively). In grade III oligodendrogliomas the Hsp27 levels  were significantly higher (p=0.03). Low MGMT expression  was associated with  grade II astrocytomas. Elevated beta-catenin expression was associated with  grade III/IV astrocytomas (p=0.003), p53 (+) tumors were more frequently found in grade III/IV astrocytomas (p=0,001). LOH on 1p was associated with oligodendroglial tumours. In addition, a higher Hsp27 expression correlated with LOH of 1p (p=0.017), this was also tested in two glioma cell lines. MSP was successful in only 6 samples. No significant correlations were found for the other markers. In conclusion, in oligodendroglial tumors Hsp27  appeared  as a surrogate marker of LOH of 1p which could also help to predict the disease  prognosis. In gliomas, p53, Hsp27, Hsp70, MGMT  and  beta-catenin correlated with histopathological characteristics suggesting that these markers could predict the disease outcome and the response to treatments.