INVESTIGADORES
GÜERCI Alba Mabel
artículos
Título:
Construction and validation of a dose-response curve using the Comet assay to determine human radiosensitivity to ionizing radiation
Autor/es:
A. GÜERCI, L. ZÚÑIGA AND R. MARCOS
Revista:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Lugar: Londres; Año: 2011 vol. 74 p. 1087 - 1093
ISSN:
1528-7394
Resumen:
Individual radiosensitivity is an individual characteristic associated with an increased reaction
to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to
classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed
by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation
in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8
Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of
the genetic damage induced by irradiation. To probe the goodness of the constructed curve,
a validation study was carried out with whole blood from two donors in a blind study. Results
show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the
interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of
the comet assay in determining individual responses to defined doses of gamma radiation.
The standard dose-response curve constructed may be used to detect individuals departing
from reference values