PERSONAL DE APOYO
MARTINEZ ZAMORA Martin Gustavo
artículos
Título:
Physiological, structural and molecular traits activated in strawberry plants after inoculation with the plant growth-promoting bacterium Azospirillum brasilense REC3
Autor/es:
GUERRERO-MOLINA, MA FERNANDA; LOVAISA, NADIA C.; SALAZAR, SERGIO M.; MARTÍNEZ ZAMORA, MARTÍN G.; DIAZ RICCI, JUAN C.; PEDRAZA, RAÚL O.
Revista:
PLANT BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014
ISSN:
1435-8603
Resumen:
The plant growth-promoting strain REC3 of Azospirillum brasilense, isolated from strawberry roots, prompts growth promotion and systemic protection against anthracnose disease on this crop. Hence, we hypothesized that A. brasilense REC3 can induce different physiological, structural and molecular responses on strawberry plants. Therefore, the aim of this work was to study these traits activated on Azospirillum-colonized strawberry plants which were not assessed until now. Healthy in vitro micropropagated plants were root inoculated with REC3 under hydroponic conditions; root and leaf tissues were sampled at different times, and oxidative burst, phenolic compounds content, malondialdehyde (MDA) concentrations, callose depositions, cell wall fortification and gene expression were evaluated. Azospirillum inoculation enhanced the levels of soluble phenolic compounds after 12 hours post-inoculation (hpi), while the amounts of cell-wall bound phenolics were similar in inoculated and control plants. Other early responses activated by REC3 (at 24 hpi) were the decline of lipid peroxidation and the up-regulation of strawberry genes involved in defense (FaPR1), bacterial recognition (FaFLS2) and H2O2 depuration (FaCAT and FaAPXc). The latter may explain the apparent absence of oxidative burst on leaves after bacterial inoculation. Also, REC3 inoculation induced delayed structural responses like callose deposition and cell wall fortification (at 72 hpi). Results showed that A. brasilense REC3 is capable of exerting beneficial traits on strawberry plants, reinforcing their physiological and cellular characteristics, which in turns contribute to improve plant performance.
