New Microsatellite loci for Prosopis alba and P. chilensis (Leguminosae)

C ECILIA F. B ESSEGA ; C AROLINA L. P OMETTI; J OE T. M ILLER; R ICHARD W ATTS; B EATRIZ O. S AIDMAN; JUAN C. VILARDI

Applications in Plant Sciences

BOTANICAL SOC AMER INC

Lugar: St. Louis; Año: 2013 p. 1 - 4

2168-0450

.Premise of the study: As only six useful microsatellite loci that exhibit broad cross-amplifi cation are so far available for Proso- pis species, it is necessary to develop a larger number of codominant markers for population genetic studies. Simple sequence repeat (SSR) markers obtained for Prosopis species from a 454 pyrosequencing run were optimized and characterized for studies in P. alba and P. chilensis . ? Methods and Results: Twelve markers that were successfully amplifi ed showed polymorphism in P. alba and P. chilensis . The number of alleles per locus ranged between two and seven and heterozygosity estimates ranged from 0.2 to 0.8. Most of these loci cross-amplify in P. ruscifolia , P. fl exuosa , P. kuntzei , P. glandulosa , and P. pallida . ? Conclusions: These loci will enable genetic diversity studies of P. alba and P. chilensis and contribute to fi ne-scale population structure, indirect estimation of relatedness among individuals, and marker-assisted selection.