Fatty Liver Is Associated with Transcriptional Downregulation of Stearoyl-CoA Desaturase and Impaired Protein Dimerization

FERNANDEZ GIANOTTI T; BURGUEÑO A,; GONZALES MANSILLA N; PIROLA CJ; SOOKOIAN S

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2013 vol. 8 p. 1 - 11

1932-6203

Aims and Methods: We evaluated the modulation of liver stearoyl-CoA desaturase-1 (Scd1) by dietary factors andinsulin resistance (IR) in two experimental models of high-fat diet (HFD)-induced nonalcoholic fatty liver disease(NAFLD). The first model included Sprague Dawley (SD) rats that developed NAFLD without IR, and the second oneincluded a rat model of genetic IR and cardiovascular disease, the spontaneously hypertensive rats (SHR) and itsnormotensive, insulin-sensitive control Wistar-Kyoto (WKY). The adult rats were given standard chow diet (CD) orHFD for 10 weeks. In all the animals, we explored the hepatic Scd1 transcriptional activity and protein levels.Results: HFD-fed rats of both strains developed severe NAFLD. Liver abundance of Scd1 mRNA was significantlydecreased in HFD-fed rats regardless of the strain; SD-CD: 235±195 vs. SD-HFD 4.5±2.9, p