INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Developing a novel computationally designed impedimetric pregabalin biosensor
Autor/es:
GHOLIVAND M; JALALVAND A; GOICOECHEA H C
Revista:
ELECTROCHIMICA ACTA
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 133 p. 123 - 131
ISSN:
0013-4686
Resumen:
A computationally designed impedimetric pregabalin (PGB) biosensor based on immobilization of bovine
serum albumin (BSA) onto graphene/glassy carbon electrode (BSA/Gr/GCE) has been developed using initial
characterization by computational methods and complementing them by experimental observations.
Computational results showed that the BSA hydrophobically binds to Gr which is energetically favorable
and leads to the spontaneous formation of the stable nanobiocomposite (BSA/Gr), and also showed that
the interaction of PGB with BSA is mainly driven by hydrogen bonding and hydrophobic interactions. The
interactions of BSA with Gr and PGB with BSA were also monitored by fluorescence and UVvis spectroscopic
techniques, and their results were consistent with the computational results. The electrochemical
properties of the fabricated composite electrodes were examined by cyclic voltammetry (CV) scanning
electron microscopy (SEM), and electrochemical impedance spectroscopy (EIS) techniques. Besides complementing
the computational studies, experimental results showed that the addition of Gr to the surface
of the electrode facilitated the electron transfer reactions, and also showed that the presence of BSA
inhibits the interfacial electron transfer in some extent due to the non-conductive properties of BSA.
The presence of the PGB may form an electroinactive complex with BSA which decelerates the interfacial
electron transfer leading to obvious faradaic impedance changes. The faradaic impedance responses
were linearly related to PGB concentration between 10.0 nM and 280.0 nM and the limit of detection
(LOD) was calculated to be 3.0 nM (3Sb/b). Finally, the proposed biosensor was successfully applied to
determination of PGB in human serum samples. The results were satisfactory and comparable to those
obtained by applying the reference method based on high performance liquid chromatography (HPLC).
The results confirmed that the proposed biosensor has good sensitivity, selectivity, stability, repeatability,
reproducibility, and regeneration ability for PGB determination