INVESTIGADORES
SAPARRAT Mario Carlos Nazareno
artículos
Título:
Ligninolytic ability and potential biotechnology applications of the south american fungus Pleurotus laciniatocrenatus
Autor/es:
SAPARRAT, M. C. N.; GUILLÉN, F.
Revista:
FOLIA MICROBIOLOGICA
Referencias:
Año: 2005 vol. 50 p. 155 - 160
ISSN:
0015-5632
Resumen:
The extracellular ligninolytic enzyme system of Pleurotus
laciniatocrenatus, grown under different culture conditions, was
characterized and the ability of this strain to degrade different
components of Eucalyptus globulus wood was determined. In shaken liquid
cultures grown on a C-limited medium supplemented with yeast extract
(0.1 %) and peptone (0.5 %), the fungus produced extracellular
aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent
peroxidase (MnP) and manganese-independent peroxidase (MiP) activities,
their maximum levels being, respectively, about 600, 50, 1360, and 920
pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 μmol/L
CuSO4 produced an increase of Lac activity levels up to
4-fold and 68.3-fold, respectively. No significant differences were
found in the levels of the other ligninolytic enzyme activities when
compared to the basal medium. Solid-state fermentation cultures on E.
globulus wood chips revealed Lac and MiP activities. These cultures
showed degradative activity on lignin and lipophilic wood extractives.