A new method for the preparation of biocompatible silica coated-collagen hydrogels

FOGLIA M LUCIA; CAMPOROTONDI DANIELA EDHIT; ALVAREZ GISELA SOLANGE; SASCHA HEINEMANN; THOMAS HANKE; CLAUDIO JAVIER PEREZ; DIAZ LUIS EDUARDO; DESIMONE MARTIN FEDERICO

JOURNAL OF MATERIALS CHEMISTRY

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2013 p. 6283 - 6290

0959-9428

Silica?collagen scaffolds were obtained by covalent binding of an aminosilane to glutaraldehyde fixedcollagen hydrogels, rendering a three dimensional network of silicon coated collagen fibrils. Whencompared to non-silicified collagen, silica containing matrices exhibited a 60 fold increment in the rheological properties. Moreover, acellular degradation by collagenase type I indicated that enzymatic digestion occurred at a slower rate for silica modified hydrogels, hence enabling a controlled degradation of the obtained material. In addition,fibroblastic cells seeded on silicified collagen matrices were able to adhere, proliferate and migrate within the scaffold for over 3 weeks as shown by MTT tests and hematoxylin?eosin staining. These results suggest that the herein described method could be useful in the design of materials for tissue engineering purposes.