INVESTIGADORES
PODHAJCER Osvaldo Luis
artículos
Título:
The multi-reference contrast method: facilitating set enrichment analysis
Autor/es:
FRESNO C; LLERA AS; GIROTTI MR; VALACCO MP; LÓPEZ JA; PODHAJCER OL; BALZARINI MG; PRADA F; FERNÁNDEZ EA
Revista:
COMPUTERS IN BIOLOGY AND MEDICINE
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2012 p. 188 - 194
ISSN:
0010-4825
Resumen:
Set enrichment analysis (SEA) is used to identify enriched biological
categories/terms within high-throughput differential expression
experiments. This is done by evaluating the proportion of differentially
expressed genes against a background reference (BR). However, the
choice of the "appropriate" BR is a perplexing problem and results will
depend on it. Here, a visualization procedure that integrates results
from several BRs and a stability analysis of enriched terms is presented
as a tool to aid SEA. The multi-reference contrast method (MRCM)
combines results from multiple BRs in a unique picture. The application
of the proposed method was illustrated in one proteomic and three
microarray experiments. The MRCM facilitates the exploration task
involved in ontology analysis on proteomic/genomic experiments, where
consensus terms were found to validate main experimental hypothesis. The
use of more than one reference may provide new biological insights. The
tool automatically highlights non-consensus terms assisting SEA