CEPAVE   05420
CENTRO DE ESTUDIOS PARASITOLOGICOS Y DE VECTORES
Unidad Ejecutora - UE
artículos
Título:
Characterization and phylogeny of Isaria spp. strains (Ascomycota: Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences
Autor/es:
CELESTE P. DALESSANDRO1, LEANDRO R. JONES, RICHARD A. HUMBER, CLAUDIA C. LÓPEZ LASTRA AND DANIEL R. SOSA-GOMEZ
Revista:
JOURNAL OF BASIC MICROBIOLOGY
Editorial:
WILEY -VERLAG
Referencias:
Lugar: WEINHEIM; Año: 2013 p. 1 - 11
ISSN:
1521-4028
Resumen:
The elongation factor 1-alpha (EF1-a) and the internal transcribed spacer (ITS) regions ITS1 and ITS2
(ITS1-5.8S-ITS2) sequences were used to characterize and to identify Isaria isolates from Argentina,
Mexico, and Brazil, aswell as to study the phylogenetic relationships among these isolates and other
related fungi fromthe order Hypocreales. Themolecular characterization, which was performed by
PCR-RFLP of EF1-a and ITS1-5.8-ITS2 genes, was useful for resolving representative isolates of Isaria
fumosorosea, Isaria farinosa, and Isaria tenuipes and to confirm the taxonomic identity of fungi from
Argentina, Mexico, and Brazil. The phylogenetic analyses showed three clades corresponding to
three families of Hypocreales. The genus Isaria was confirmed as polyphyletic and in family
Cordycipitaceae, Isaria species were related to anamorphic species of Beauveria, Lecanicillium, and
Simplicillium and to teleomorphic Cordyceps and Torrubiella. Therefore,EF1-a and ITS1-5.8S-ITS2 genes
were found to be powerful tools for improving the characterization, identification, and phylogenetic
relationship of the Isaria species and other entomopathogenic fungi.