INVESTIGADORES
GOICOECHEA Hector Casimiro
artículos
Título:
Fabrication of an ultrasensitive impedimetric buprenorphine hydrochloride biosensor from computational and experimental angles
Autor/es:
GHOLIVAND M; JALALVAND A; GOICOECHEA H C; SKOV T
Revista:
TALANTA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2014
ISSN:
0039-9140
Resumen:
For the first time, an ultrasensitive impedimetric buprenorphine hydrochloride (BN)
biosensor based on immobilization of bovine serum albumin (BSA) onto multi-walled carbon
nanotubes (MWCNTs)/glassy carbon electrode (BSA/MWCNTs/GCE) has been developed using initial
characterization by computational methods and complementing them by experimental observations.
Computational results showed that the BSA hydrophobically binds to MWCNTs which is energetically
favorable and leads to spontaneous formation of the stable BSA/MWCNTs nanobiocomposite
(bioconjugate). Computational results also showed that the interaction of BN with BSA is mainly driven
by hydrophobic interactions. The interactions of BSA with MWCNTs and BN with BSA were also
monitored by fluorescence and UV-Vis spectroscopic techniques, and their results were consistent with
the computational results. Morphology and electrochemical properties of the fabricated composite
electrodes were examined by scanning electron microscopy (SEM), cyclic voltammetry (CV), and
electrochemical impedance spectroscopy (EIS). Besides complementing the computational studies,
experimental results showed that the addition of MWCNTs to the surface of the GCE greatly facilitated
the electron transfer reactions, and also showed that the presence of BSA inhibits the interfacial
electron transfer in some extent due to the non-conductive properties of BSA. On the other hand, the
presence of BN may form an electroactive complex with BSA which accelerates the interfacial electron
transfer and leads to obvious faradaic impedance changes. The faradaic impedance responses were
linearly related to BN concentration between 5.0 nM and 72.0 nM and a limit of detection (LOD, 3Sb/b)
of 1.5 nM was achieved. Finally, the proposed biosensor was successfully applied to determination of
BN in urine samples of both healthy and addict volunteers. The results were satisfactory and
comparable to those obtained by applying the reference method based on high performance liquid
chromatography-ultraviolet detection (HPLC-UV). It is expected that the distinctive features of
BSA/MWCNTs nanobiocomposite would make it potentially advantageous for a broad range of
biosensing, and clinical applications.