INVESTIGADORES
FABRA Adriana Isidora
artículos
Título:
Genetic diversity of phosphate-solubilizing peanut (Arachis hypogaea L.) associated bacteria and mechanisms involved in this ability
Autor/es:
ANZUAY, M.; FROLA,O.; ANGELINI, J.; LUDUEÑA, L; FABRA, ADRIANA; TAURIAN, T.
Revista:
SYMBIOSIS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 60 p. 143 - 154
ISSN:
0334-5114
Resumen:
Abstract In this study, attempts were made to analyze mechanisms
involved in the bacterial phosphate-solubilizing ability
of peanut isolates. Bacteria were taxonomically identified by
analysis of 16S rDNA sequence. Levels of soluble P released
by the isolates in unbuffered or buffered with Tris?HCl or
MES NBRIP-BPB medium as well as the production of Dgluconic
acid were determined in their culture. Presence of
two of the genes encoding the cofactor PQQ of GDH enzyme
was analyzed in the genome of this bacterial collection. 16S
rDNA sequence analysis indicated that isolates belong to
genera Serratia, Enterobacter, Pantoea, Acinetobacter,
Bacillus and Enterococcus. All bacteria showed ability to
solubilize tricalcium phosphate either in unbuffered or buffered
medium. Nevertheless, addition of buffer solutions reduced
levels of Pi liberated by the isolates. Although almost
all isolates produced detectable amounts of D-gluconic acid,
no correlation with levels of P soluble released were observed.
The presence of pqqE and pqqC genes was detected only in
Gram negative bacteria. It was concluded from this study that
themechanisminvolved in phosphate solubilization is organic
acids production and, presence of pqq genes in all Gram
negative bacteria analyzed encourages to confirm their role
in bacterial phosphate solubilizing ability as well to identify
genes involved in this PGP trait in Gram positive bacteria