INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Rat Lefty2/EBAF gene: Isolation and expression analysis in the oviduct during the early pregnancy stage
Autor/es:
MARTIN E. ARGAÑARAZ; PABLO A. VALDECANTOS; CARLOS M. ABATE; DORA C. MICELI
Revista:
Genes & Genetic Systems
Referencias:
Año: 2007 vol. 82 p. 145 - 156
ISSN:
1341-7568
Resumen:
It has been demonstrated,by RNA Arbitrarily Primed Polymerase Chain Reac-
tion (RAP-PCR),that the endometrial bleeding associated factor (ebaf or lefty2 )
was expressed in rat oviduct.In this work we isolated and sequenced the full-
length left2 cDNA from Rattus norvegicus oviducts and described its expression
level in this organ during the estrous cycle and early pregnancy stage.The cod-
ing deduced sequence (CDS)codifies a 40.91 kDa protein with a highly conserved
TGF-â functional domain.RT-PCR semiquantitative analyses indicated that ovi-
duct cells transcribe lefty2 among different stages of the estrous cycle with the
maximum expression at diestrus phase.The highest expression of lefty2 was at
the 4 th day after mating (five folds respect to day one),just when the embryos have
completed their transit through the oviduct.The lefty2 expression declined rap-
idly thereafter and the levels of their transcripts in the oviduct remained low until
7 th days after mating.