INVESTIGADORES
GOMEZ-MEJIBA sandra Esther
artículos
Título:
IMMUNO-SPIN TRAPPING OF PROTEIN AND DNA RADICALS
Autor/es:
SANDRA E GOMEZ-MEJIBA; ZHAI, Z.; AKRAM, H.; DETERDING, LJ; TOMER, KB; MASON, RP; HENSLEY, K.; RHEAL TOWNER; MASON, RP; RAMIREZ DC
Revista:
FREE RADICAL BIOLOGY AND MEDICINE
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2009 vol. 46 p. 853 - 865
ISSN:
0891-5849
Resumen:
Biomolecule-centered radicals are intermediate species produced during
both reversible (redox modulation) and irreversible (oxidative stress)
oxidative modification of biomolecules. These oxidative processes must
be studied in situ and in real time to understand the molecular
mechanism of cell adaptation or death in response to changes in the
extracellular environment. In this regard, we have developed and
validated immuno-spin trapping to tag the redox process, tracing the
oxidatively generated modification of biomolecules, in situ and in real
time, by detecting protein- and DNA-centered radicals. The purpose of
this methods article is to introduce and update the basic methods and
applications of immuno-spin trapping for the study of redox biochemistry
in oxidative stress and redox regulation. We describe in detail the
production, detection, and location of protein and DNA radicals in
biochemical systems, cells, and tissues, and in the whole animal as
well, by using immuno-spin trapping with the nitrone spin trap
5,5-dimethyl-1-pyrroline N-oxide.