Cytochrome P450 expression and function in liver and intestinal mucosa from dexamethasone-treated sheep.

MATÉ, L.; LIFSCHITZ, A.; SALLOVITZ, J.; BALLENT, M.; MUSCHER A.; WILKENS M.; SCHRODER B.; LANUSSE, C.; VIRKEL, G.

JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2011 p. 1 - 8

0140-7783

The effects of repeated administrations of dexamethasone (DEX) (3 mg/kg/day by IM route for 7 days) on the gene expression profile of a cytochrome P450 (CYP) 3A28-like isoenzyme, on the expression of a CYP3A-immunoreactive protein and on CYP3A-dependent metabolic activities in sheep liver and small intestinal mucosa were evaluated in the current work. CYP 3A-dependent metabolic activities (erythromycin and triacetyl-oleandomycin N-demethylations) were assessed in microsomal fractions. The mRNA expression of CYP3A28-like, glucocorticoid receptor, constitutive androstane receptor, pregnane X receptor and retinoic X receptor alpha (RXRá) was determined by quantitative real-time PCR. The expression of a CYP3A-immunoreactive protein was measured by Western blot analyses. In the liver, DEX treatment increased CYP3A28-like mRNA levels (2.67-fold, P