Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease

RODRÍGUEZ FM; SALVETTI NR; COLOMBERO M; STANGAFERRO ML; BARBEITO CG; ORTEGA HH; REY F

ANIMAL REPRODUCTION SCIENCE

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 140 p. 14 - 25

0378-4320

Cystic ovarian disease (COD) is one of the main factors responsible for reproductive23 disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not24 fully understood, it has been proposed that the IGF system could play an essential role, as25 it is a key intraovarian regulator. The aim of the present study was to determine whether26 the altered levels in IGF1 detected in bovines with COD are associated with changes at27 mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF28 components studied were analyzed by real time PCR and in situ hybridization, and IGFBP29 expression and activity were assayed by immunohistochemistry and ligand blot30 respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell31 gene expression in cystic follicles (P < 0.05). Results also showed variations in IGFBP32 expression in the intraovarian cellular compartment and concentration in follicular fluid,33 and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.