INVESTIGADORES
HOZBOR Daniela Flavia
artículos
Título:
Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection
Autor/es:
BOTTERO D; GAILLARD ME; ERREA A; MORENO G; ZURITA E; PIANCIOLA L; RUMBO M; HOZBOR D
Revista:
VACCINE
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2013 p. 1 - 9
ISSN:
0264-410X
Resumen:
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Bordetella parapertussis, a close
related species of B. pertussis, can
also cause the disease named pertussis or whooping cough. The number of cases
caused by this related pathogen has risen sustained in the last years. The
widely used cellular (wP) or acellular (aP) pertussis vaccines have little or
no efficacy against B. parapertussis. In
an effort to devise an effective acellular vaccine against B. parapertussis infection, outer membrane vesicles (OMVs) were
obtained from B. parapertussis.
Proteomic analysis of the resulting OMVs, designated OMVsBpp, evidenced the
presence of several surface immunogens including pertactin. The characterized
OMVsBpp were used in murine B.
parapertussis intranasal challenge model to examine their protective
capacity when administered by systemic route. Immunized BALB/c mice were
challenged with sublethal doses of B.
parapertussis. Significant differences between immunized animals and the
negative control group were observed (p < 0.001). OMVsBpp protected against B. parapertussis infection, whereas
current commercial aP vaccine showed little protection against such pathogen.
More interestingly, protection induced by OMVsBpp against B. pertussis was comparable to our previously designed vaccine
consisting in OMVs derived from B.
pertussis (OMVsBp). For these experiments we used as a positive control the
current commercial aP vaccine in high dose. As expected aP offered protection
against B. pertussis in mice.
Altogether the results presented here showed that the OMVs from B. parapertussis are an attractive
vaccine candidate to protect against whooping cough induced by B. parapertussis but also by B. pertussis.
