INVESTIGADORES
HOZBOR Daniela Flavia
artículos
Título:
Quantitation of adenylate cyclase of Bordetella pertussis by enzyme linked immunoabsorbent assay
Autor/es:
HOZBOR D; CHIRDO F; RODRIGUEZ ME; VALVERDE C; YANTORNO O
Revista:
BIOLOGICALS
Editorial:
ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD
Referencias:
Año: 1995 p. 279 - 284
ISSN:
1045-1056
Resumen:
Bordetella pertussis produces extracytoplasmic adenylate cyclase toxin
(AC) which has received considerable attention as a potential vaccine
candidate. Great interest from laboratories involved in production,
purification and quality control of acellular pertussis vaccine is
focused on finding an appropriate technique for rapid and accurate
quantitation of AC antigen. In this paper a competitive ELISA is
proposed. A polystyrene microplate coated with purified AC was incubated
with the sample to be tested plus anti-AC serum. The bound anti-AC
antibodies were measured by sequential reaction with alkaline
phosphatase-labelled anti-mouse IgG and p-nitrophenylphosphate. This
method showed high specificity, with the 50% inhibition corresponding to
4 micrograms/ml of AC. It also proved to be useful to assess the
presence of AC in culture supernatants, with high reproducibility.