INVESTIGADORES
ORTEGA Hugo Hector
artículos
Título:
Intermediate Filament Proteins Expression and Carbohydrate Moieties in Trophoblast and Decidual Cells of Mature Cat Placenta
Autor/es:
FERNANDEZ, PE; DIESSLER, ME; PACHAME, A; ORTEGA HH; GIMENO EJ; PORTIANSKY, EL; BARBEITO, CG
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013
ISSN:
0936-6768
Resumen:
The aim of this study was to characterize cytoskeletalintermediate filament proteins and glycoconjugates of syncytiumtrophoblast,cytotrophoblast and decidual cells of felineendotheliochorial placenta. Samples from 12 normal pregnantfemale cats, after 45 5 days of gestation, were obtainedremoving the uterine horns by hysterectomy. Sections wereprocessed for routine observation and for immunohistochemistryusing anticytokeratin, antivimentin and antidesmin antibodies.In addition, lectin histochemistry was performed usinga panel of several biotinylated lectins to characterize glycosidesexpression profile. Cytotrophoblast and syncytiotrophoblastshowed immunoreactivity only with acidic and basic cytokeratins.Decidual cells were only positive to vimentin, consistentwith their origin from endometrial fibroblasts. Trophoblastexpressed a broad population of glycans, highly exposingterminal N-acetyl glucosamine residues and non-sialylatedgalactose and N-acetyl galactosamine oligomers. Oligosaccharidesbound by Phaseolus vulgaris erythroagglutinin were theonly highly branched N-linked residues evidenced in cats, andthey were restricted to the syncytium. Unlike results reportedon humans, mice and rats on lectin affinity of decidual cells,sialid acids and complex N-linked oligosaccharides were notdemonstrated in cats. Glycosylation of proteins determinesmany of their final properties, thus becoming essential for theembryo-maternal dialogue during implantation and placentation.Changes in glycosylation pattern have been related topathological pregnancies in other species. Hence, the knowledgeabout glycosylation profile of the normal cat placentamay lead to a better understanding of both normal andpathological reproductive events.
