Lectin-binding pattern in ovarian structures of rats with experimental polycystic ovaries

BARBEITO, CG; ORTEGA HH; MATILLER, V; GIMENO EJ; SALVETTI NR

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 vol. 48 p. 850 - 857

0936-6768

Numerous experimental models in different species have beendeveloped for the study of polycystic ovarian syndrome. Inthis study, we used a model of induction of polycystic ovaries(PO) in rats by exposure to constant light to study thedistribution and variations of glycosylated residues present inthe different ovarian structures. Seven biotinylated lectins wereused (Con-A, WGA, DBA, SBA, PNA, RCA and UEA-I) ontissue sections, and detection was performed using the streptavidin/peroxidase method. In tissue sections was observed anincrease in affinity for Con-A in the granulosa and thecainterna of growing follicles and cysts in animals with PO in5 relation to the control group. Follicular cysts showed higheraffinity for WGA and RCA-I which growing follicles in thesame group, and there was a decrease in affinity for PNA in thecysts in relation to the growth of follicles in both groups.Atretic follicles in both groups showed greater labelling withlectins PNA, SBA and RCA-I in relation to healthy follicles. Itcould also be noted that the zona pellucida of cystic follicleslost the affinity for the lectin Con-A. There was no staining onfollicles in any category with the lectins DBA and UEA-I,although it was staining in the corpus luteum (control group)and in the mesothelium and interstitial glands of both groupswith DBA. These observations probably reflect changes in theglycosaminoglycans present in the different ovarian compartmentsor in the glycosylation of cellular components essentialfor proper follicular dynamics.