INVESTIGADORES
GONZALEZ Ana Maria
artículos
Título:
Direct shoot regeneration from leaf and internode explants of Aloysia polystachya [Gris.] Mold. (Verbenaceae).
Autor/es:
L. BURDYN; C. LUNA; J. TARRAGO; P. SANSBERRO; N. DUDIT; A. GONZALEZ; L. MROGINSKI
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Referencias:
Año: 2006 p. 235 - 239
ISSN:
1054-5476
Resumen:
Adventitious bud regeneration from leaf and internode explants of Aloysia polystachya was achieved. Shoots from nodal
segments grown in vitro were cut into pieces and used as sources of explants. Organogenesis was induced from both explants
cultured on quarter-strength Murashige and Skoog (MS) semisolid medium (plus sucrose 5 g l21) containing different
combinations of 6-benzyladenine (BA) and a-naphthalenacetic acid (NAA) under 116 mmolm22 s21 photosynthetic photon
flux density (PPFD), 14-h photoperiod, and at a temperature of 27 ^ 28C. The type of explant markedly influenced
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
flux density (PPFD), 14-h photoperiod, and at a temperature of 27 ^ 28C. The type of explant markedly influenced
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
combinations of 6-benzyladenine (BA) and a-naphthalenacetic acid (NAA) under 116 mmolm22 s21 photosynthetic photon
flux density (PPFD), 14-h photoperiod, and at a temperature of 27 ^ 28C. The type of explant markedly influenced
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
flux density (PPFD), 14-h photoperiod, and at a temperature of 27 ^ 28C. The type of explant markedly influenced
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
An experimental field plot with 2-yr-old in vitro-regenerated plants was established.
organogenesis and growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants, while the
number of shoots formed per responsive explant was greater with internode explants. However, the growth of regenerated
shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant
increased from one to seven as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA at
0.05mM in combination with BA at 0.5 mM induced the highest regeneration rate (87 ^ 8.8%) after 20 d of culture, yielding
5.9 ^ 0.8 shoots per responsive leaf explant. Histological examination confirmed the occurrence of direct organogenesis. The
regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for
30 d. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA at 500mM for 2 h and
transferred to 1/4 MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions.
An experimental field plot with 2-yr-old in vitro-regenerated pla