CIPYP   05508
CENTRO DE INVESTIGACIONES SOBRE PORFIRINAS Y PORFIRIAS
Unidad Ejecutora - UE
artículos
Título:
Heme oxygenase activity and oxidative stress signaling in soybean leaves
Autor/es:
BALESTRASSE KARINA; NORIEGA GUILLERMO; BATLLE ALCIRA; TOMARO MARIA LUJAN
Revista:
PLANT SCIENCE
Referencias:
Año: 2006 vol. 170 p. 339 - 346
ISSN:
0168-9452
Resumen:
We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for soybean plants against
oxidative stress. Here, we have investigated for the first time the possible signal transduction pathways involved in heme oxygenase-1 induction in
leaves of soybean plants. Treatment with 200 mM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased
70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin
(protein phosphatases inhibitor), LaCl3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, guanylate cyclase
inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2, which were again
prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by
pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress,
triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship
between oxidative stress generation and heme oxygenase induction in higher plants.mM Cd during 48 h increased 87% thiobarbituric acid reactive substances, whereas GSH decreased
70%, respect to controls. These effects were prevented by preincubation with diphenyleneiodonium (DPI, NADPH oxidase inhibitor), cantharidin
(protein phosphatases inhibitor), LaCl3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, guanylate cyclase
inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2, which were again
prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by
pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress,
triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship
between oxidative stress generation and heme oxygenase induction in higher plants.3 (calcium channel blocker) and [1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ, guanylate cyclase
inhibitor). Two hundred micromoles Cd produced increased concentrations and in situ accumulation of H2O2 and O2, which were again
prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by
pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress,
triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship
between oxidative stress generation and heme oxygenase induction in higher plants.2O2 and O2, which were again
prevented by DPI, cantharidin, LaCl3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by
pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress,
triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship
between oxidative stress generation and heme oxygenase induction in higher plants.3 and ODQ. Moreover, Cd-induced heme oxygenase-1 activity was also totally or partially abolished by
pretreatment with the different inhibitors. These results clearly demonstrated that the signal transduction pathways involved in oxidative stress,
triggered by cadmium ions were similar to those implicated in heme oxygenase-1 induction, and supported the proposal of a close relationship
between oxidative stress generation and heme oxygenase induction in higher plants.