INVESTIGADORES
PRECIADO Maria Victoria
artículos
Título:
Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children
Autor/es:
GISMONDI MI; DIAZ CARRASCO, J. M.; VALVA P; BECKER, P.; GUZMÁN, C.; CAMPOS, R.; MARIA VICTORIA PRECIADO
Revista:
VIROLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 447 p. 187 - 196
ISSN:
0042-6822
Resumen:
Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2 to 9.6 years of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability. Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure.