INVESTIGADORES
GONZALEZ Daniel Hector
artículos
Título:
A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria
Autor/es:
GONZALEZ, DANIEL H.; BONNARD, GERALDINE; GRIENENBERGER, JEAN-MICHEL
Revista:
CURRENT GENETICS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 1993 vol. 24 p. 248 - 255
ISSN:
0172-8083
Resumen:
Sequence analysis of a transcribed region of the wheat mitochondrial
(mt) genome revealed two open reading frames (orfs) coding for proteins
of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb
RNA. The largest orf codes for a hydrophobic protein which bears
similarity to a bacterial protein involved in the biogenesis of c-type
cytochromes. Its corresponding RNA sequence is fully edited at 34
positions. The second orf encodes a protein homologous to the
amino-terminal third of E. coli ribosomal protein S1, corresponding to
the ribosome-binding domain of this protein. Its RNA sequence is edited
at four positions, one of the edits creating a stop codon. The presence
of both proteins in wheat mitochondria was demonstrated using specific
antibodies raised against fusion proteins obtained in E. coli from the
corresponding cDNAs.