INVESTIGADORES
BARBEITO Claudio Gustavo
artículos
Título:
Antimitotic activity of EA21b mammary-carcinoma extract
Autor/es:
FLAMINI MA; GONZÁLEZ NV; BARBEITO CG
Revista:
BIOLOGIA
Editorial:
Springer
Referencias:
Lugar: Bratislava; Año: 2007 vol. 62 p. 1 - 5
ISSN:
0142-3355
Resumen:
Many tumors produce factors that affect cell-cycle and cell proliferation. In the present study we have analyzed
the effect of a mammary-tumor extract injection on the mitotic activity of several organs in young male C3H/S mice
previously standardized for circadian periodicity. One-half of the animals received an intraperitoneal EA21b tumor extract
dose at 16:00 h, while the other half received saline. Animals were sacrificed on the following day at 08:00, 12:00 or 16:00 h,
4 h after receiving an injection of colchicine by the same route. Samples of duodenum, kidney, liver, and submaxillary gland
were excised and processed for hematoxylin-eosin staining. Mitotic indices, expressed as the number of colchicine-arrested
metaphases per 1,000 nuclei, were assessed in convoluted tubule epithelium, duodenal crypt enterocytes, hepatocytes and
submaxillary gland ductal and acinar sialocytes. All values were expressed as mean ± SEM. Statistical analyses were
performed by ANOVA, Bonferroni and Student?s t-tests. In contrast to the mitotic indices reductions observed in renal
convoluted tubules cells and duodenal crypt enterocytes, neither the submaxillary gland nor the liver were found to contain
cell types whose mitotic activity was affected by the tumor extract. We conclude that EA21b mammary carcinoma contains
one or more factors that inhibit the proliferation of selected populations of normal cells.
± SEM. Statistical analyses were
performed by ANOVA, Bonferroni and Student?s t-tests. In contrast to the mitotic indices reductions observed in renal
convoluted tubules cells and duodenal crypt enterocytes, neither the submaxillary gland nor the liver were found to contain
cell types whose mitotic activity was affected by the tumor extract. We conclude that EA21b mammary carcinoma contains
one or more factors that inhibit the proliferation of selected populations of normal cells.