Characterization of Cytoskeletal Proteins in Follicular Structures of Cows with Cystic Ovarian Disease

ORTEGA H; SALVETTI N; AMABLE P; DALLARD B; BARAVALLE C; BARBEITO CG; GIMENO EJ

JOURNAL OF COMPARATIVE PATHOLOGY

Elsevier

Lugar: Bristol, UK; Año: 2007 vol. 136 p. 222 - 230

0021-9975

The distribution of intermediate ¢laments (vimentin, cytokeratins, desmin) and micro¢laments (a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin andmuscle speci¢c actin)was studied immunohistochemically inbovine ovaries,with andwithout cystic ovarian disease.The immunohistochemically stained area (IHCSA), was quanti¢ed by image analysis, to evaluate the expression of these cytoskeletal proteins in the follicular wall of healthy antral, atretic, and cystic follicles. The granulosa cell layer of cystic follicles and atretic folliclus had a signi¢cantly larger IHCSA for vimentin than did healthy antral follicles. Cytokeratins reacted lightly in the granulosa cells of antral follicles of normal ovaries, whereas granulosa cells of atretic and cystic follicles showed signi¢cantly higher IHCSA values. Immunohistochemical localization of desmin, muscle speci¢c actin, and a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. a-smooth muscle actin was restricted to the theca externa. This study supports earlier suggestions that strongly positive reactions with vimentin and cytokeratins antibodies observed in the granulosa cells of cystic follicles are due to the reorganization that occurs in the follicle during the process of cystic development, and are associatedwith changes in the expression of cytoskeletal proteins that are essential to proper cellular functioning. r