Effects of presence of tumors on the daily mitotic activity of mouse pars intermedia

SURUR JM; CATALANO VA; FLAMINI MA; BARBEITO CG

CELL BIOLOGY INTERNATIONAL

Elsevier

Lugar: Aberdeen, UK; Año: 2005 vol. 29 p. 173 - 175

1065-6995

We have investigated the effects of EA21a and EA34 mammary carcinomas on daily PI cell proliferation in mice. Animals were divided into groups grafted with either EA34 or EA21a carcinomas (and a non-grafted control group). They were all injected intraperitoneally with 2 mg colchicine per g of body weight 4 h before sacrifice and the number of mitoses per 1000 nuclei was calculated. The mitotic index (MI) of pars-intermedia epithelial cells in control animals showed significant temporal variations. However, the MI from mice grafted with EA34 or EA21a carcinomas showed no such variation. There was no difference between the daily MIs of controls and tumor grafted groups. The absence of a 24 h mitotic activity curve in both EA21a and EA34 tumorbearing animals demonstrates a lower level of synchronization of cells entering mitosis.
2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved