INVESTIGADORES
LO NOSTRO Fabiana Laura
artículos
Título:
Expression of pejerrey gonadotropin-releasing hormone (pjGnRH) in the three orders of fish
Autor/es:
G. SOMOZA; D. LESCHEID; L. MIRANDA; F. LO NOSTRO; I. MAGLIULO-CEPRIANO; A. MONTANER; M. SCHREIBMAN; J. RIVIER; N. SHERWOOD
Revista:
BIOLOGY OF REPRODUCTION
Editorial:
Society for the Study of Reproduction,
Referencias:
Lugar: USA; Año: 2002 vol. 67 p. 1864 - 1871
ISSN:
0006-3363
Resumen:
Molecular variants of GnRH were characterized by reversephase,
high-performance liquid chromatography from brain extracts
of fish in three different orders: Synbranchiformes (swamp
eel [ Synbranchus marmoratus]), Cyprinidontiformes (platyfish
[ Xiphophorus maculatus] and green swordtail [ X. helleri]), and
Atheriniformes (Patagonia pejerrey [ Odontesthes hatchery]).
Also, pituitary gland extracts from the pejerrey O. bonariensis
(Atheriniformes) were characterized. Eluted fractions were tested
in radioimmunoassays with antisera specific to GnRH, including
both antisera that detected only one form of GnRH and
those that detected several forms. The results show that brain
extracts obtained from all species contained the same three molecular
forms of GnRH, which were immunologically and chromatographically
undistinguishable from chicken GnRH-II, pejerrey
GnRH (pjGnRH), and salmon GnRH. This study supports the
hypothesis that expression of these three forms is common in
different fish orders and that pjGnRH is the main regulator of
pituitary function in these fish