CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Post-ischemic environmental enrichment protects the retina from ischemic damage in adult rats
Autor/es:
DORFMAN D; FERNANDEZ DC; CHIANELLI MS; MIRANDA M; ARANDA ML; ROSENSTEIN RE
Revista:
EXPERIMENTAL NEUROLOGY
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2013 vol. 240 p. 146 - 156
ISSN:
0014-4886
Resumen:
Exp Neurol. 2013 Feb;240:146-56. doi: 10.1016/j.expneurol.2012.11.017. Epub 2012 Nov 27.
Post-ischemic environmental enrichment protects the retina from ischemic damage in adult rats.
Dorfman D, Fernandez DC, Chianelli M, Miranda M, Aranda ML, Rosenstein RE.
Source
Laboratory
of Retinal Neurochemistry and Experimental Ophthalmology, Department of
Human Biochemistry, School of Medicine, University of Buenos
Aires/CEFyBO, CONICET, Buenos Aires, Argentina.
Abstract
The
aim of this study was to elucidate whether post-ischemic enriched
environment (EE) housing protects the retina from ischemic damage in
adult rats, and the involvement of glutamate in retinal protection
induced by EE housing. For this purpose, ischemia was induced by
increasing intraocular pressure to 120 mm Hg for 40 min. After ischemia,
animals were housed in a standard environment (SE) or EE and subjected
to electroretinography and histological analysis. EE housing afforded
significant functional protection in eyes exposed to
ischemia/reperfusion injury. A marked reduction in retinal thickness and
ganglion cell number, and an increase in Müller cell glial fibrillary
acidic protein (GFAP) levels were observed in ischemic retinas from
SE-housed animals, which were reversed by EE housing. A deficit in
anterograde transport from the retina to the superior colliculus was
observed in SE- but not in EE-housed animals. In SE-housed animals,
ischemia induced a significant decrease in retinal glutamate uptake and
glutamine synthetase activity, whereas EE housing reversed the effect of
ischemia on these parameters. The intravitreal injection of
supraphysiological levels of glutamate partially reproduced retinal
alterations induced by ischemia/reperfusion, which were abrogated by EE
housing. These results indicate that EE housing significantly protected
retinal function and histology from ischemia/reperfusion injury in adult
rats, likely through a glutamate-dependent mechanism.