CERZOS   05458
CENTRO DE RECURSOS NATURALES RENOVABLES DE LA ZONA SEMIARIDA
Unidad Ejecutora - UE
artículos
Título:
Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
Autor/es:
CURVETTO, NÉSTOR; FIGLAS, DÉBORA; BRANDOLIN, ADRIANA; SAIDMAN, SILVANA; RUEDA, HAYDEÉ; FERREIRA, MARÍA LUJÁN
Revista:
BIOCHEMICAL ENGINEERING JOURNAL
Editorial:
Elsevier
Referencias:
Lugar: London; Año: 2006 vol. 29 p. 191 - 203
ISSN:
1369-703X
Resumen:
The presentwork analyzes the potential use of white-rot fungi (WRF) and hematin for phenol and aniline polymerization, as a low-cost alternative
to horseradish peroxidase (HRPC). The objective is to evaluate the capability of these catalysts to produce tailor-made aniline as well as to eliminate
phenols by precipitation from aqueous solution. 4-Aminoantypirine (4AAP) was used to test phenoxide formation by crude protein preparations
of white-rot fungi at selected conditions. The crude extracts of Pleurotus sajor-caju (PSC) were selected because of the promising values obtained
for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
Pleurotus sajor-caju (PSC) were selected because of the promising values obtained
for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the
presence of polystyrene sulphonated (PES), with high aniline conversions at 45 ◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.
◦C. For the case of insoluble polyphenol production, the PSC-derived
enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one
step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversionwas obtained when using PSC enzymes
at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline
(PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from
wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
© 2005 Elsevier B.V. All rights reserved.