Imaging molecular interactions in living cells by FRET

ELIZABETH A JARES-ERIJMAN AND THOMAS M JOVIN

CURRENT OPINION IN CHEMICAL BIOLOGY.

Año: 2006 vol. 10 p. 409 - 416

1367-5931

Foerster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a ‘nanoruler’ with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function. The complex underlying network of interactions reflects elementary reactions operating under strict spatio-temporal control: binding, conformational transition, covalent modification and transport. FRET imaging provides information about all these molecular processes with high specificity and sensitivity via probes expressed by or introduced from the external medium into the cell, tissue or organism. Current approaches and developments in the field are discussed with emphasis on formalism, probes and technical implementation.