INVESTIGADORES
VIDOZ Maria Laura
artículos
Título:
Plant regeneration of Lotononis bainesii Baker (Fabaceae) through cotyledon and leaf culture.
Autor/es:
MARÍA LAURA VIDOZ; KENNETH HAYS QUESENBERRY; DANIEL REAL; MARIA GALLO
Revista:
AFRICAN JOURNAL OF BIOTECHNOLOGY
Editorial:
ACADEMIC JOURNALS
Referencias:
Lugar: New York; Año: 2012 vol. 11 p. 9724 - 9731
ISSN:
1684-5315
Resumen:
Lotononis
bainesii Baker is a promising perennial forage legume
for subtropical regions. Development of
tissue culture methods for in vitro plant regeneration could facilitate crop
improvement methods. Plant regeneration
was obtained for over 50% of L. bainesii
cv. INIA Glencoe genotypes evaluated via cotyledon culture and 90% of genotypes
evaluated by leaflet culture in a medium composed of MS + 4.5 µM thidiazuron (TDZ).
Bud elongation and rooting were obtained upon transfer onto Murashige and Skoog
(MS) + 0.044 mM
6-benzyladenine (BA) + 0.049 mM
indolebutyric acid (IBA). Although immature leaflet culture resulted in a
higher number of responsive genotypes than cotyledon culture, plants
regenerated from cotyledons exhibited a higher survival rate when transferred
to ex vitro conditions. Culture
medium supplementation with either Picloram (PIC) or 2,4-dichlorophenoxyacetic
acid (2,4-D) resulted in friable callus, that did not grow when subcultured.
Immature leaflet insertion areas (petiole tip area where the three leaflets are
attached) from expanding leaves and pieces of immature leaflets were the most
efficient explants for shoot bud induction. A recommended protocol for L. bainesii plant regeneration would be
placing immature leaflet explants on MS + 4.5 µM TDZ for 30 days; followed by
transfer onto MS + 0.044 mM
BA + 0.049 mM
IBA for bud elongation and rooting.