INVESTIGADORES
FONSECA Maria Isabel
artículos
Título:
Chips and sawdust substrates application for lignocellulolytic enzymes production by solid state fermentation
Autor/es:
ERNESTO MARTÍN GIORGIO, MARÍA ISABEL FONSECA, MARCOS RAÚL TEJERINA, ANA BELÉN RAMOS-HRYB, NOELIA SANABRIA, PEDRO DARÍO ZAPATA, LAURA LIDIA VILLALBA
Revista:
International Research Journal of Biotechnology
Editorial:
International Research Journals Publishing House
Referencias:
Lugar: Sapale; Año: 2012 vol. 7 p. 120 - 127
ISSN:
2141-5153
Resumen:
White rot fungi have an enzymatic system producing o
xidative and hydrolytic enzymes that act on the
degradation of the cell wall components. The abunda
nce of sawdust and other forestry wastes in our
region, due to an important forestry activity, repr
esents an interesting ecological option. These surp
lus
needs to be ecologically disposed. The purpose of t
his study was to compare wood chips and sawdust
as substrates for the production of hydrolytic (end
o-
β
-1,4-glucanase and 1,4-
β
-glucosidase) and
oxidative (laccases and manganese peroxidase) enzyme
s secreted by five white rot fungi native from
Misiones (Argentina) by solid state fermentation (S
SF) We also evaluated the effect of the extraction
method on enzyme recovery yield. Sawdust-SSF was pro
ved to be the most suitable substrate for
enzymes production.
Endo-
β
-1,4-glucanase and laccase were the major enzymes se
creted with both
substrates.
Peniophora
sp.
BAFC 633,
T. villosa
BAFC 2755 and
P. sanguineus
BAFC 2126 were the
most efficient producers of both types of enzymes.
G. applanatum
BAFC 1168 strain F and
C. versicolor
f.
antarcticus
BAFC 266 produced mostly hydrolytic enzymes. Enzyme e
xtraction was conducted in two
steps to assure optimal recovery yield. A second ex
traction step was necessary to sawdust SSF
samples.