Adsorption and electrooxidation of dna at glassy carbon electrodes modified with multi-wall carbon nanotubes dispersed in glucose oxidase.

FABIANA A. GUTIERREZ; MARÍA D. RUBIANES; GUSTAVO A. RIVAS

ELECTROANALYSIS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 25 p. 1135 - 1142

1040-0397

We are proposing for the first time the successful immobilization of DNA at glassy carbon electrodes (GCE) modified with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorption or by layer-by-layer self-assembling using polydiallyldimethylamine (PDDA). The presence of GOx allows an efficient dispersion of CNT and gives a most favorable environment that promotes the adsorption and makes possible a more sensitive electrooxidation of DNA. The PDDA incorporated in the self-assembled architecture largely facilitates the adsorption and electrooxidation of dsDNA and the adsorbed layer can be successfully used for evaluating the interaction of DNA with methylene blue.