In vitro regeneration of Fragaria vesca’s plants from leaf and petiole explants

HAEL CONRAD, VERÓNICA; TOMAS GRAU, RODRIGO H.; DÍAZ RICCI, JUAN CARLOS

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Tucumán; Año: 2012

0327-9545

To develop a genetic transformation technique it is essential to have an efficient regeneration system. Thus, the objective of this work was to adjust a plant regeneration system from leaf and petiole explants of local accessions of F. vesca. Fruits were collected in Villa Nougués, achenes were extracted, scarified with sulfuric acid and plated on two media cultures: half-strength MS 4% sucrose (MS1) and MS 3% sucrose (MS2) (both pH 5.74). Cultures were maintained at 25 ± 2 °C and 16 hs light photoperiod (light intensity: 40 μmoles/m2.s). After 4 months MS1 medium was chosen since these plants were larger. Leaf and petiole seccions were excised from 4-months-old seedlings and cultured on regeneration medium (MS with 3% sucrose, 3 mg/I BAP and 0.25 mg/l IBA for leaves, and 1 mg/I BAP and 0.25 mg/l IBA for petioles, pH 5.74). Leaves and petioles regenerated as masses of undifferentiated cells called callus. 70% of leaves callus and 50% of petioles callus gave shoots. 5 mm-height-shoots were excised and cultured in MS1. Rooted plants were rusticated into sterile peat, kept in humid chamber under standard growth conditions and one month later placed in greenhouse. In conclusion, we selected a suitable culture medium for in vitro germination of achenes of F. vesca, and adjusted an efficient plant regeneration system from leaf and petiole explants.