INVESTIGADORES
RODRIGUEZ VIRASORO Ramiro Esteban
artículos
Título:
The Flavoenzime Ferredoxin (Flavodoxin)-NADP(H) Reductase Modulates NADP(H) Homeostasis During the soxRS Response of Escherichia coli
Autor/es:
ADRIANA KRAPP; RAMIRO ESTEBAN RODRIGUEZ VIRASORO; HUGO POLI; DARÍO PALADINI; JAVIER PALATNIK; NESTOR CARRILLO
Revista:
JOURNAL OF BACTERIOLOGY
Referencias:
Año: 2002 vol. 184 p. 1474 - 1480
ISSN:
0021-9193
Resumen:
Escherichia coli cells from strain fpr, deficient in the soxRS-induced ferredoxin (flavodoxin)-NADP(H) reductase (FPR), display abnormal sensitivity to the bactericidal effects of the superoxide-generating reagent methyl viologen (MV). Neither bacteriostatic effects nor inactivation of oxidant-sensitive hydrolyases could be detected in fpr cells exposed to MV. FPR inactivation did not affect the MV-driven soxRS response, whereas FPR overexpression led to enhanced stimulation of the regulon, with concomitant oxidation of the NADPH pool. Accumulation of a site-directed FPR mutant that uses NAD(H) instead of NADP(H) had no effect on soxRS induction and failed to protect fpr cells from MV toxicity, suggesting that FPR contributes to NADP(H) homeostasis in stressed bacteria.
