Room-temperature fluorescence spectroscopy of monohydroxy metabolites of polycyclic aromatic hydrocarbons on octadecyl extraction membranes

CALLIMAG, K; GOICOECHEA, H C; A CAMPIGLIA,

TALANTA

ELSEVIER SCIENCE BV

Año: 2011 vol. 85 p. 1805 - 1811

0039-9140

Urine analysis of monohydroxy metabolites is recognized as an accurate assessment of humanexposure to polycyclic aromatic hydrocarbons. Despite the sophisticated arsenal of analyticaltools, monitoring of monohydroxy metabolites via simple, cost effective and direct methods ofanalysis still remains a challenge. This article evaluates the analytical potential of solid-phaseextraction room-temperature fluorescence spectroscopy for the problem at hand. Extractionmembranes serve the dual purpose of sample pre-concentration and solid substrate for RTFmeasurements. The potential of our proposition is demonstrated with the analysis of 2-hydroxyfluorene,1-hydroxy-pyrene, 3-hydroxy-benzo[a]pyrene and 9-hydroxy-phenanthrene insynthetic urine samples. Signal reproducibility is improved with the aid of a sample holderspecifically designed for the manual optimization of luminescence signals. Backgroundcorrection of solid substrates is carried out with the aid of Asymmetric Least Squares. Recoveryvalues for the studied metabolites varied from 99.0 ± 1.2% (3-hydroxy-benzo[a]pyrene) to 99.9± 0.05% (1-hydroxy-pyrene). With only 10mL of urine sample, the limits of detection variedfrom 57pg.mL-1 (2-hydroxy-fluorene) to 2pg.mL-1 (1-hydroxy-pyrene). Additional figures ofmerit include a simple experimental procedure for routine screening of numerous samples andcompatibility with portable instrumentation for field analysis. Because of the non-destructivenature of fluorescence measurements, membranes can be brought to the lab for subsequentelution and confirmation of compounds via high-resolution techniques.