Bioremediation of metsulfuron methyl by Agaricus blazei. Bioremediation Journal

• GONZÁLEZ MATUTE, R., FIGLAS, D. AND CURVETTO, N.

BIOREMEDIATION JOURNAL

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2012 vol. 16 p. 31 - 37

1088-9868

Metsulfuron methyl (MM) is an herbicide used in cereal crops. Agaricus blazei Murrill is a white rot fungi which is also an important edible and medicinal mushroom, reported to be a major laccase producer, a lignin degrading enzyme with low substrate specificity. To demonstrate the potential application of A. blazei spent mushroom compost (SMC) in the cleaning of soils polluted with MM, a phytotoxic dose of MM was incubated with two enzyme preparations obtained from A. blazei SMC after the second fruiting flush and the resulting extracts mixtures were then used in a plant growth test with Brassica napus L. plantlets. The whole enzyme preparation (I) or a partially purified extract (II) and their dilutions, 1:10 and 1:100, were incubated in the presence of MM (5 10-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.Agaricus blazei Murrill is a white rot fungi which is also an important edible and medicinal mushroom, reported to be a major laccase producer, a lignin degrading enzyme with low substrate specificity. To demonstrate the potential application of A. blazei spent mushroom compost (SMC) in the cleaning of soils polluted with MM, a phytotoxic dose of MM was incubated with two enzyme preparations obtained from A. blazei SMC after the second fruiting flush and the resulting extracts mixtures were then used in a plant growth test with Brassica napus L. plantlets. The whole enzyme preparation (I) or a partially purified extract (II) and their dilutions, 1:10 and 1:100, were incubated in the presence of MM (5 10-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.A. blazei spent mushroom compost (SMC) in the cleaning of soils polluted with MM, a phytotoxic dose of MM was incubated with two enzyme preparations obtained from A. blazei SMC after the second fruiting flush and the resulting extracts mixtures were then used in a plant growth test with Brassica napus L. plantlets. The whole enzyme preparation (I) or a partially purified extract (II) and their dilutions, 1:10 and 1:100, were incubated in the presence of MM (5 10-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.A. blazei SMC after the second fruiting flush and the resulting extracts mixtures were then used in a plant growth test with Brassica napus L. plantlets. The whole enzyme preparation (I) or a partially purified extract (II) and their dilutions, 1:10 and 1:100, were incubated in the presence of MM (5 10-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.Brassica napus L. plantlets. The whole enzyme preparation (I) or a partially purified extract (II) and their dilutions, 1:10 and 1:100, were incubated in the presence of MM (5 10-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.-3 ppm) at 25°C during 24, 48, 72 and 96 hours. When the hypocotyl length of plantlets was measured, extracts I and II with MM incubated for 72 and 96 hours, showed a highly significant increase with respect to the obtained with plantlets exposed to MM alone. Results show the ability that easily extracted enzyme fraction from A. blazei SMC has to degrade MM, decreasing its phytotoxicity.A. blazei SMC has to degrade MM, decreasing its phytotoxicity.