Susceptibility of beneficial lactic acid bacteria and potential pathogens to antimicrobial agents used in ranicultura

G. MONTEL MENDOZA; S.E. PASTERIS; M.I. BÜHLER; M.E. NADER-MACÍAS

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2012 vol. 36 p. 81 - 81

0327-9545

The design of probiotics for raniculture requires evaluating their functional properties such as resistance to chemotherapeutics frequently used for treatment/prevention of diseases in Lithobates catesbeianus hatcheries. The susceptibility of probiotic candidates’ lactic acid bacteria (LAB) (Lactococcus lactis CRL1584, CRL1827; L. garvieae CRL1828; Lactobacillus plantarum CRL1606 and Enterococcus gallinarum CRL1826) to antibiotic and antiseptics used in raniculture was studied but also the antiseptics susceptibility of pathogenic bacteria: Pseudomonas aeruginosa, Citrobacter freundii and Listeria monocytogenes. The minimal inhibitory concentration (MIC) was determined according to the CLSI recommendations. The LAB strains were susceptible to most of the antibiotics, but they were metronidazole resistant. CRL1584 and CRL1606 strains were oxytetracycline resistant, and CRL1606 and 1828 strains vancomycin resistant while CRL1826 (ceftazidime resistant) presents the vancomycin resistance genes, being susceptible in phenotypic assays. The antiseptics MIC values of LAB and pathogens were higher than the concentrations used routinely in hatcheries. The results indicate that L. lactis CRL1584 and 1827 could be included in a probiotic product. Although the pathogens showed resistance to antiseptics, they were also sensitive to LAB antimicrobial metabolites.