Performance improvement of araujiain, a cysteinphytoprotease, by immobilization within calcium alginate beads

E. QUIROGA; C. ILLANES; N.A. OCHOA; S. BARBERIS

PROCESS BIOCHEMISTRY

ELSEVIER

Año: 2011 vol. 46 p. 1029 - 1034

0032-9592

The effect of immobilization within alginate beads on the performance of araujiain in aqueous and non-aqueous media was studied.Optimum pH and temperature, thermal and pH-stability, kinetic parameters and operational stability were determined. Entrapped enzyme showed an increase in thermal and pH-stability,  a high activity over the studied pH range (5.0?9.5), and a shiftin optimum temperature from 37 ◦C to70◦C. Immobilized araujiain retained  95% of the irinitial activity after 45days of storage at 4 ◦C, and itmaintained 78% of activity after 20 hydrolytic reaction cycles.Furthermore, it showed a good performance in non-aqueous medium, used for the peptide synthesis of interest for the food industry, with a yield 2.6-fold greater than using free enzyme. The entrapped enzyme was more stable over a wide range of temperatures, pH, and storage time as compared to free enzyme.