INVESTIGADORES
GAY Claudia Carolina
artículos
Título:
Synergism between baltergin metalloproteinase and Ba SPII RP4 PLA2 from Bothrops alternatus venom on skeletal muscle (C2C12) cells
Autor/es:
BUSTILLO, S.; GAY, C.C.; GARCÍA DENEGRI, M.E.; PONCE-SOTO, L.A.; BAL DE KIER JOFFÉ, E. ; ACOSTA, O.C.; LEIVA, L.C.
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Glasgow; Año: 2012 vol. 59 p. 338 - 343
ISSN:
0041-0101
Resumen:
Acute muscle damage, myonecrosis, is one of the main characteristics of envenoming byBothrops genus. In this in vitro study we investigated the role of a metalloproteinase(baltergin) and an acidic phospholipase A2 (Ba SPII RP4) in the cytotoxicity exhibited byBothrops alternatus venom. Baltergin metalloproteinase purified from the venom exerteda toxic effect on C2C12 myoblast cells (CC50: 583.34 µg/mL) which involved morphological alterations compatible with apoptosis/anoikis. On the contrary, the most abundant PLA2 isolated from this venom did not exhibit cytotoxicity at times and doses tested. However, when myoblasts were treated with both enzymes together, synergic activity was demonstrated. Neutralization of the venom with specific antibodies (IgG anti-baltergin and IgG anti-PLA2) confirmed this synergism.
