INVESTIGADORES
ALVAREZ Hector Manuel
artículos
Título:
Identification of phenyldecanoic acid as constituent of triacylglycerols and wax ester produced by Rhodococcus opacus PD630
Autor/es:
ALVAREZ, H. M.; LUFTMANN H,; SILVA RA,; CESARI AC,; VIALE A,; WÄLTERMANN M,; STEINBÜCHEL A,
Revista:
MICROBIOLOGY-UK
Editorial:
Society for General Microbiology (UK)
Referencias:
Lugar: UK; Año: 2002 vol. 148 p. 1407 - 1412
ISSN:
1350-0872
Resumen:
Phenyldecane supported growth and lipid accumulation of Rhodococcus
opacus PD630 during cultivation under nitrogen-limiting conditions. The
results of this study suggested that the hydrocarbon phenyldecane was
degraded by monoterminal oxidation, followed by b-oxidation of the alkyl
side-chain to phenylacetic acid, and by an additional degradative route for the
oxidation of the latter to intermediates of the central metabolism. á-Oxidation
of phenyldecanoic acid also occurred to some extent. Phenyldecanoic acid, the
monoterminal oxidation product, was also utilized for the biosynthesis of a
novel wax ester and novel triacylglycerols. The formation of the wax ester
phenyldecylphenyldecanoate probably resulted from the condensation of
phenyldecanoic acid and phenyldecanol, which were produced as metabolites
during the catabolism of phenyldecane. Two types of triacylglycerol were
detected in phenyldecane-grown cells of strain PD630. Triacylglycerols
containing only odd- and even-numbered aliphatic fatty acids, as well as
triacylglycerols in which one fatty acid was replaced by a phenyldecanoic acid
residue, occurred. Other phenyl intermediates, such as phenylacetic acid,
phenylpropionic acid, 4-hydroxyphenylpropionic acid, protocatechuate and
homogentisic acid, were excreted into the medium during cultivation on
phenyldecane. On the basis of the results obtained, pathways for the
catabolism and assimilation of phenyldecane by R. opacus PD630 are discussed.