INVESTIGADORES
GIOVAMBATTISTA Andres
artículos
Título:
Direct Effect of Ghrelin on Leptin Production
Autor/es:
GIOVAMBATTISTA, ANDRÉS; PIERMARÍA, JUDITH; SUESCUN, MARÍA OLGA; CALANDRA, RICARDO SAÚL; GAILLARD, ROLF; SPINEDI, EDUARDO
Revista:
OBESITY RESEARCH
Editorial:
North American Association for the Study of Obesity
Referencias:
Lugar: United States; Año: 2006 vol. 14 p. 19 - 27
ISSN:
1071-7323
Resumen:
Abstract
GIOVAMBATTISTA, ANDRE´ S, JUDITH PIERMARI´A,
MARI´A O. SUESCUN, RICARDO S. CALANDRA,
ROLF C. GAILLARD, AND EDUARDO SPINEDI. Direct
effect of ghrelin on leptin production by cultured rat white
adipocytes. Obesity. 2006;14:19 27.Obesity. 2006;14:19 27.
Objective: Because ghrelin is known to stimulate adipogenesis,
we tested whether ghrelin could contribute to the
maintenance of homeostasis, directly affecting rat white
adipocyte leptin production.
we tested whether ghrelin could contribute to the
maintenance of homeostasis, directly affecting rat white
adipocyte leptin production.
Because ghrelin is known to stimulate adipogenesis,
we tested whether ghrelin could contribute to the
maintenance of homeostasis, directly affecting rat white
adipocyte leptin production.
Research Methods and Procedures: Isolated retroperitoneal
adipocytes were cultured for 0.5 to 48 hours without
(baseline) or with (0.001 to 1 nM) ghrelin alone or in
combination with insulin (0.01 to 10 nM) or dexamethasone
(1 to 100 nM). Adipocytes were also incubated with ghrelin
and inhibitors either of RNA (actinomycin D) or protein
synthesis (cycloheximide) or with several concentrations
(10 to 1000 nM) of a specific ghrelin antagonist. When
cultures were terminated, we evaluated adipocyte leptin
secretion and ob mRNA expression.
adipocytes were cultured for 0.5 to 48 hours without
(baseline) or with (0.001 to 1 nM) ghrelin alone or in
combination with insulin (0.01 to 10 nM) or dexamethasone
(1 to 100 nM). Adipocytes were also incubated with ghrelin
and inhibitors either of RNA (actinomycin D) or protein
synthesis (cycloheximide) or with several concentrations
(10 to 1000 nM) of a specific ghrelin antagonist. When
cultures were terminated, we evaluated adipocyte leptin
secretion and ob mRNA expression.
Isolated retroperitoneal
adipocytes were cultured for 0.5 to 48 hours without
(baseline) or with (0.001 to 1 nM) ghrelin alone or in
combination with insulin (0.01 to 10 nM) or dexamethasone
(1 to 100 nM). Adipocytes were also incubated with ghrelin
and inhibitors either of RNA (actinomycin D) or protein
synthesis (cycloheximide) or with several concentrations
(10 to 1000 nM) of a specific ghrelin antagonist. When
cultures were terminated, we evaluated adipocyte leptin
secretion and ob mRNA expression.ob mRNA expression.
Results: Our data indicate that ghrelin directly enhanced
adipocyte leptin release and ob mRNA expression, that the
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
adipocyte leptin release and ob mRNA expression, that the
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
Our data indicate that ghrelin directly enhanced
adipocyte leptin release and ob mRNA expression, that the
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
ob mRNA expression, that the
leptin-releasing activity of ghrelin was additive to the action
of both insulin and dexamethasone and was abrogated by
protein synthesis inhibitors, and that effects of ghrelin on
adipocyte ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
ob mRNA expression and release were blocked
by coincubation with the specific growth hormone secretagogue
receptor 1a antagonist.
Discussion: Our study supports the ability of ghrelin to
enhance white adipose tissue leptin production by a direct
receptor-mediated effect. This activity of ghrelin could play
a potentially significant role in rapid restoration of homeostasis
after food intake.
enhance white adipose tissue leptin production by a direct
receptor-mediated effect. This activity of ghrelin could play
a potentially significant role in rapid restoration of homeostasis
after food intake.
Our study supports the ability of ghrelin to
enhance white adipose tissue leptin production by a direct
receptor-mediated effect. This activity of ghrelin could play
a potentially significant role in rapid restoration of homeostasis
after food intake.
Key words: GHS-R1a, ghrelin-A, ob mRNA expression,
insulin, dexamethasone
insulin, dexamethasone
ob mRNA expression,
insulin, dexamethasone