Respuesta inmune humoral contra una bacterina de staphylococcus aureus cp5 en vaquillonas empleando dos vías de inoculación

CAMUSSONE CECILIA; SIGNORINI M; GALARZA R; REJF PAULA; MARCIPAR IVÁN; CALVINHO LUIS

Revista Fave Ciencias Veterinarias

Facultatad de Agronomía y Veterinarioa de la UNL

Año: 2010 vol. 9 p. 51 - 58

1666-938X

Staphylococcus aureus is the most frequently isolated pathogen from bovine chronic intramammary infections in Argentina. This organism produces capsular polysaccharides (CP) and antibodies directed against these components favour opsonophagocytosis. The aim of this study was to evaluate the humoral immune response against a S. aureus bacterin of a reference strain CP5 type (Reynolds) using two different inoculation routes. Eighteen Holstein pregnant heifers were used. One group was vaccinated subcutaneously in the supramammary lymph node area (SLNA), a second group subcutaneously in the neck and a third group was a non vaccinated control. Levels of total IgG were assessed in blood sera and whey by an ELISA technique. Vaccinated animals showed significantly higher IgG levels than non vaccinated controls. In addition, heifers vaccinated through the SLNA route showed the highest IgG levels in blood sera; while IgG levels in whey were higher in vaccinates than controls, although differences between inoculation routes were not detected.