Survival of heat stress with and without heat hardening in Drosophila melanogaster: interactions with larval density

ARIAS L N; SAMBUCETTI P; SCANNAPIECO A C; LOESCHCKE V; NORRY F M

JOURNAL OF EXPERIMENTAL BIOLOGY

COMPANY OF BIOLOGISTS LTD

Lugar: Cambridge; Año: 2012 vol. 215 p. 2220 - 2225

0022-0949

Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila  melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and  RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening  treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E–16F6; 30A3–34C2; 49C–50C). Previously found thermotolerance QTL were also significant for flies from HD cultures.