INSIBIO   05451
INSTITUTO SUPERIOR DE INVESTIGACIONES BIOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Rat lefty/ebaf gene: isolation and expression analysis in the oviduct during early pregnancy stage
Autor/es:
ARGAÑARAZ MARTÍN; VALDECANTOS PABLO; ABATE CARLOS; MICELI DORA C.
Revista:
Genes and genetics systems
Editorial:
Genetics Society of Japan
Referencias:
Lugar: Japón; Año: 2006 vol. #MS6
ISSN:
1341-7568
Resumen:
Rat Lefty2/EBAF Gene:Isolation and Expression
Analysis in the Oviduct During the
Early Pregnancy Stage
Argañaraz,Martin E.1 ,Valdecantos,Pablo A.1 ,Abate,Carlos M.2
and Miceli Dora C.1 *
1 Instituto Superior de Investigaciones Biológicas.INSIBIO (CONICET-Universidad Nacional
de Tucumán),Chacabuco 461,4000.S.M.de Tucumán,Argentina
2 Planta Piloto de Procesos Industriales Microbiológicos.PROIMI (CONICET),Av.
Belgrano y Pje.Caseros,4000.S.M.de Tucumán,Argentina
(Received 3 November 2006,accepted 26 February 2007)
It has been demonstrated,by RNA Arbitrarily Primed Polymerase Chain Reac-
tion (RAP-PCR),that the endometrial bleeding associated factor (ebaf or lefty2 )
was expressed in rat oviduct.In this work we isolated and sequenced the full-
length left2 cDNA from Rattus norvegicus oviducts and described its expression
level in this organ during the estrous cycle and early pregnancy stage.The cod-
ing deduced sequence (CDS)codifies a 40.91 kDa protein with a highly conserved
TGF-â functional domain.RT-PCR semiquantitative analyses indicated that ovi-
duct cells transcribe lefty2 among different stages of the estrous cycle with the
maximum expression at diestrus phase.The highest expression of lefty2 was at
the 4 th day after mating (five folds respect to day one),just when the embryos have
completed their transit through the oviduct.The lefty2 expression declined rap-
idly thereafter and the levels of their transcripts in the oviduct remained low until
7 th days after mating.