INVESTIGADORES
BATTAGLINI Fernando
artículos
Título:
Use of an Antimicrobial Protein for Endotoxin Detection in a Competitive Electrochemical Assay.
Autor/es:
G. PRIANO; F. BATTAGLINI
Revista:
ANALYTICAL CHEMISTRY
Editorial:
American Chemical Society
Referencias:
Lugar: Washington; Año: 2005 vol. 77 p. 4976 - 4984
ISSN:
0003-2700
Resumen:
Endotoxins, also referred to as pyrogens, are part of the
cellular walls of Gram-negative bacteria and are capable
of inducing fever when entering into the blood stream.
Current methods of detection involve the use of either
amoebocytes from horseshoe crab that clots upon exposure
to endotoxin or a live assay on rabbits. In this work,
the detection of endotoxins by an electrochemical competitive
assay is presented. Two configurations of modified
electrodes were constructed using a recombinant endotoxin
neutralizing protein (ENP) as recognition element.
A modified lipopolysaccharide with horseradish peroxidase
was used for the competitive assay. Modified electrodes
constructed by electrostatic interaction of ENP and
an electroactive polymer can detect the presence of
endotoxins in concentrations as low as 0.2EU mL-1,
below the limit imposed for water in injectable drugs in
the American Pharmacopoeia. Modified electrodes constructed
by covalent linking of ENP to a carboxymethyl
dextran matrix bound to the electrode show a better
dynamic range but a higher detection limit.-1,
below the limit imposed for water in injectable drugs in
the American Pharmacopoeia. Modified electrodes constructed
by covalent linking of ENP to a carboxymethyl
dextran matrix bound to the electrode show a better
dynamic range but a higher detection limit.