INVESTIGADORES
LUX Victoria Adela R.
artículos
Título:
Sol-gel immobilized ovarian follicles: collaboration between two different cell types in hormone production and secretion.
Autor/es:
CATALANO, P. N; BOURGUIGNON N.; ÁLVAREZ, C.; LIBERTUN, C.; DÍAZ, L. E.; DESIMONE, M. F.; LUX LANTOS, V.
Revista:
JOURNAL OF MATERIALS CHEMISTRY
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Lugar: CAMBRIDGE; Año: 2012 vol. 22 p. 11681 - 11687
ISSN:
0959-9428
Resumen:
The immobilization of mammalian cells within semipermeable matrices which allow retention of
viability and functionality has recently attracted research interest. In this study, rat mature ovarian
follicles were encapsulated in solgel silica matrices for the evaluation of in vitro steroid hormones
production. Tetraethoxysilane (TEOS) and tetrakis(2-hydroxyethyl)orthosilicate (THEOS) were
evaluated for cell viability, progesterone and estradiol secretion and cell structure conservation.
Significant differences were observed between TEOS and THEOS, which allowed encapsulation of
follicles for 9 days. Although encapsulated follicles showed lower viability and proliferation rates than
control follicles, they were able to secrete estradiol to the same extent as control follicles. This study
demonstrated an effective collaboration between two different mammalian cell types in hormone
production and secretion when encapsulated in silica matrices. These results could lead to the
development of ovarian cell-based hormone delivery systems for the treatment of the symptoms of
menopause and the Turner syndrome minimizing adverse effects.
production. Tetraethoxysilane (TEOS) and tetrakis(2-hydroxyethyl)orthosilicate (THEOS) were
evaluated for cell viability, progesterone and estradiol secretion and cell structure conservation.
Significant differences were observed between TEOS and THEOS, which allowed encapsulation of
follicles for 9 days. Although encapsulated follicles showed lower viability and proliferation rates than
control follicles, they were able to secrete estradiol to the same extent as control follicles. This study
demonstrated an effective collaboration between two different mammalian cell types in hormone
production and secretion when encapsulated in silica matrices. These results could lead to the
development of ovarian cell-based hormone delivery systems for the treatment of the symptoms of
menopause and the Turner syndrome minimizing adverse effects.
in vitro steroid hormones
production. Tetraethoxysilane (TEOS) and tetrakis(2-hydroxyethyl)orthosilicate (THEOS) were
evaluated for cell viability, progesterone and estradiol secretion and cell structure conservation.
Significant differences were observed between TEOS and THEOS, which allowed encapsulation of
follicles for 9 days. Although encapsulated follicles showed lower viability and proliferation rates than
control follicles, they were able to secrete estradiol to the same extent as control follicles. This study
demonstrated an effective collaboration between two different mammalian cell types in hormone
production and secretion when encapsulated in silica matrices. These results could lead to the
development of ovarian cell-based hormone delivery systems for the treatment of the symptoms of
menopause and the Turner syndrome minimizing adverse effects.